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By Amitava Dasgupta

Эта серьёзная книга рассказывает о роли антиоксидантов для нашего здоровья. bankruptcy 1 advent to loose Radicals and the Body’s Antioxidant protection bankruptcy 2 tools for Measuring Oxidative rigidity within the Laboratory bankruptcy three Oxidative pressure brought on via pollution and publicity to daylight bankruptcy four Oxidative tension because of Cigarette Smoking, Alcohol Abuse, and Drug Abuse bankruptcy five Oxidative pressure triggered by means of family chemical substances bankruptcy 6 mental Stress-Induced Oxidative rigidity bankruptcy 7 Oxidative pressure and Cardiovascular illnesses bankruptcy eight Oxidative rigidity and melanoma bankruptcy nine Diabetes and Oxidative tension bankruptcy 10 function of Oxidative tension in Neurodegenerative ailments and different ailments regarding getting older bankruptcy eleven Oxidative tension regarding different ailments bankruptcy 12 end result, greens, and Nuts bankruptcy thirteen Tea, espresso, and Chocolate: wealthy resources of Antioxidants bankruptcy 14 Alcoholic drinks: Antioxidant and different health and wellbeing merits of reasonable intake bankruptcy 15 Antioxidant nutrients bankruptcy sixteen natural and different supplements which are Antioxidants bankruptcy 17 scuffling with Oxidative pressure with a fit way of life

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Glutathione and various antioxidant vitamins, such as vitamin C, vitamin E, and various carotenoids, are also measured to evaluate the effect of oxidative stress in both human and animal studies. Assay kits are commercially available for analysis of glutathione. For example, in the assay kit from 35 36 CHAPTER 2: M e t ho ds f o r M ea s ur i ng O x i da t iv e S t r e s s i n t h e L a b o r a t o r y SigmaÀAldrich, the sample is first deproteinized with the 5% 5-sulfosalicylic acid solution and the glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of DTNB to TNB.

The authors commented that oxidation of LDL can also be 23 24 CHAPTER 2: M e t ho ds f o r M ea s ur i ng O x i da t iv e S t r e s s i n t h e L a b o r a t o r y initiated by copper ions. Instead of using LDL oxidation in vitro, oxidation of methyl linoleate in vitro in the presence of peroxyl radicals generated by decomposition of an azo compound can be used to measure antioxidant capacity of food or natural product extract. This assay is called the inhibition of linoleic acid in vitro assay [10].

When an antioxidant reduces copper (II) neocuproine to copper (I) neocuproine, a blue color develops with absorption maxima at 450 nm. The intensity of the blue color is related to the antioxidant capacity of the compound tested. This method can be applied for determination of antioxidant capacity of food constituents. However, in addition to determining antioxidant capacity of polyphenols and flavonoids, this method can be used for measuring antioxidant capacity of human serum. Using ammonium acetate buffer medium for the reaction, total antioxidant capacity of serum due to ascorbic acid, α-tocopherol, and glutathione can be directly estimated by recording absorbance at 450 nm.

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